Search results for "genetic polyphasic approach"

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A taxonomic survey of lactic acid bacteria isolated from wheat (Triticum durum) kernels and non-conventional flours

2007

In order to explore the correspondence between raw material- and mature sourdough-lactic acid bacterial (LAB) communities, 59 Italian wheat (Triticum durum) grain samples, one bran and six non-conventional flour samples were analyzed through a culture-dependent approach. The highest cell count by an agar medium specific for LAB was 2.16 log CFU/g. From about 2300 presumptive LAB (Gram-positive and catalase-negative) colonies collected, a total of 356 isolates were subjected to identification by a genetic polyphasic strategy consisting of RAPD-PCR analysis, partial 16S rRNA gene sequencing, species-specific and multiplex PCRs. The isolates were recognized as 137 strains belonging to Aerococc…

DNA BacterialDietary FiberLactococcusEnterococcus mundtiiFlourMolecular Sequence Dataculture-dependent niethods genetic polyphasic approach lactic acid bacteria non-conventional flours sourdough Triticum durumColony Count MicrobialGram-Positive BacteriaApplied Microbiology and BiotechnologyMicrobiologyDNA RibosomalMicrobiologyLactobacillusRNA Ribosomal 16SSequence Homology Nucleic Acidmetodi coltura-dipendenti approccio polifasico genetico impasti acidiFood scienceLactic AcidEcology Evolution Behavior and SystematicsPhylogenyTriticumgenetic polyphasic approachsourdoughbiologyfood and beveragesGenes rRNASequence Analysis DNAbiology.organism_classification16S ribosomal RNACatalaseDNA FingerprintingRandom Amplified Polymorphic DNA Techniquelactic acid bacteriaRNA BacterialEnterococcusItalyTriticum durumAerococcusPediococcusEdible Grainnon-conventional floursculture-dependent niethodsEnterococcus faecium
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